Eppendorf BioSpectrometer basic Manual de usuario descargar pdf (Pagina 87)

Technical data

Eppendorf BioSpectrometer

basic

English (EN)

11.6 Application parameters

Methods Preprogrammed and freely programmable methods for all measuring

and evaluation procedures:

• Absorbance measurements with one or more wavelengths, scans

• Nucleic acids and proteins, OD600, dye methods (parallel

measurement of biomolecule and dye marking)

• Methods with evaluation via factor, standard and standard series

• Dual wavelength procedure with subtraction and division

evaluation

Method-dependent evaluation Absorbance, concentration via factor and standard.

Concentration via standard series:

• Linear regression

• Nonlinear regression (2nd and 3rd degree polynoms)

• Spline evaluation

• Linear interpolation (point-to-point evaluation)

Absorbance calculations via subtraction and division

Additional data for nucleic acids: Ratios 260/280 and 260/230; molar

concentration, total yield

Additional data for dye methods: FOI (frequency of incorporation,

labeling density)

Scans: zoom, peak evaluation

Method memory >100 method programs

Measured value memory and

calibration memory

Memory for >1 000 results with all data of the results evaluation and

standard evaluation, sample number, sample name, date and used

parameter set of the method program.

(The number of saved results depends on the number of saved

methods.)

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Eppendorf BioSpectrometer basic Manual de usuario Pagina 87