Eppendorf BioSpectrometer basic Manual de usuario descargar pdf (Pagina 53)

Methods

Eppendorf BioSpectrometer

basic

English (EN)

More calculations

Press the [More calc.] softkey.

Nucleic acids method group:

• After the molar mass has been entered (in base/

base pairs or in kDa): Convert the concentration

result to the molar concentration.

• After the sample volume has been entered:

Calculate the total amount in the sample.

Dye labels method group:

Nucleic acid:

• After the molar mass has been entered (in base/

base pairs or in kDa): Convert the concentration

result to the molar concentration.

• After the sample volume has been entered:

Calculate the total amount in the sample.

Dye:

• After entering the volume of the sample:

Calculate the total amount in the sample.

• For dsDNA the calculation of the molar concentration is based on the assumption of a

double-stranded nucleic acid. For the ssDNA, RNA and Oligo methods, a single-stranded

nucleic acid is assumed.

• For methods which have been reprogrammed via <New Method> in the Routine main

group, Nucleic acids method group, always double-stranded nucleic acids are assumed

for calculating the molar concentration.

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Eppendorf BioSpectrometer basic Manual de usuario Pagina 53