Eppendorf BioSpectrometer basic Manual de usuario descargar pdf (Pagina 54)

Methods

Eppendorf BioSpectrometer

basic

English (EN)

Peak detection

Press the [Peaks] softkey. For the peak detection you can alternate between two criteria:

• λ grid: Evaluation grid on the wavelength scale for the peak detection (e.g., 10 nm).

10 nm example: The spectra section from -5 nm to +5 nm is evaluated in relation to the peak to be

detected.

• Min. Δ Abs: Minimum difference between the peak to be detected and the lowest absorbance in the

evaluation grid. No absorbance value in the grid may be higher than the value of the peak at any given

time (e.g.: 0.5).

Examples:

λ grid: 100 nm, min. Δ Abs: 0.050:

The peak is not detected because the λ grid is too

large: The absorbance values on the left edge of the

grid are higher than the absorbance of the peak.

λ grid: 20 nm, min. Δ Abs: 0.200:

The peak is not detected because the predetermined

value for min. Δ abs is too high. The difference of the

absorbance of the peak and the lowest absorbance in

the grid is less than 0.2 A.

λ grid: 20 nm, min. Δ Abs: 0.050:

The peak is detected.

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Eppendorf BioSpectrometer basic Manual de usuario Pagina 54